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In the above mentioned experiments, the extrapolation from the reaction factor did not consider the dependence with the instrument response on chain duration, nor the diploma as well as posture of unsaturations. Liu et al.14 formulated a method to quantify VLC-PUFA expressed as fatty acids molar proportion, though the reaction of a single quadrupole mass analyzer exhibits developments attributable to chain length, number of unsaturations and their situation, and the necessity of doing extrapolations.
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Table one shows the uncertainties for every stage of calibration curve calculated since the arithmetic indicate from 8 calibration curves comparable to Each and every PUFA methyl ester reference conventional obtainable (see Fig. S1), with distinctive quantity of carbons, amount of unsaturations and unsaturation positions. The precision of your calibration curves received from the chosen PUFA requirements and The shortage of traits in the response of QTOF as a consequence of lengthy chain or unsaturations, confirmed the potential for the quantitation of VLC-PUFA with acyl chains longer than 24 carbons, steering clear of as significantly as possible biases as a result of discrepancies among the molecule buildings.
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